TAMPA, Fla. 鈥� The often costly, time-consuming process of obtaining blood work and urinalysis could soon get a major overhaul. Researchers at the 麻豆视频 in Tampa have created a mobile version of the 鈥淓nzyme Linked Immunosorbent Assay鈥� (ELISA), the gold standard technique used to detect the presence of an antibody or antigen. Instead of sending patients to a laboratory, new cell phone-based technology allows for the very same test to be conducted in the doctor鈥檚 office, clinic or even in a remote area.
鈥淓LISA is an important technology for biochemical analysis of proteins and hormones and is critical for the diagnosis of many diseases, such as HIV and Lyme disease,鈥� said corresponding author Anna Pyayt, PhD, assistant professor at the 麻豆视频 College of Engineering. 鈥淏ut the machines required for the incubation and reading are expensive and bulky. The MELISA allows patients to undergo testing and obtain results at point-of-care.鈥�
The Mobile Enzyme Linked Immunosorbent Assay (MELISA) is detailed in in Biosensors and Bioelectronics. The device accurately measures progesterone levels, a key hormone that impacts female fertility and is indicative of some cancers. MELISA consists of a water bath heater that incubates samples at a target temperature and analyzes them via images taken by mobile phone. It uses color analysis to determine the RGB (red, green, blue) color components of each sample. The blue color component is used for further analysis due to its sensitivity to the changes in progesterone concentration.
鈥淚t is designed to make biomedical testing simple and affordable. When low-cost testing can be integrated with routine clinic visits, this would greatly improve the quality of health care and detect worrisome signs earlier,鈥� Dr. Pyayt said.
The portable MELISA weighs just one pound, dramatically helping older patients suffering chronic conditions and those across the world who have difficulty getting to clinical labs. Dr. Pyayt and her team at USF are now calibrating MELISA for a variety of tests including testosterone. With those results, they鈥檒l apply for FDA approval so clinics can start using the device.